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As anticipated, this Ni II -catalyzed alcoholysis followed by simple one-pot hydrazinolysis can release peptide hydrazides in moderate to good yields [ 63 ]. In addition to protein side-chain modifications, acyl hydrazone chemistry has also been found useful for protein linear assembly [ 9 , 10 ], terminal modification [ 11 ] and cyclization [ 12 ]. Chemical synthesis of proteins using hydrazide intermediates. They have implemented several security measures, including two-factor authentication and a whitelisting process.
